Researchers Rewire Intracellular Signals for Use in Reengineering Biological Processes-Non-cross reacting protein-protein interfaces

The illustrations show the computational design of non-cross reacting protein-protein interfaces from fixed (bottom left) and flexible (right) backbone modeling compared to the wild-type complex (top left). Small backbone changes allowed the side chains of R56 and E1373 to adopt conformations that can form hydrogen bonds (dashed lines). Key: gray is Cdc42; teal is ITSN; the five designed interface residues are shown in sticks.

NOTE: ILLUSTRATION IS EXTRACTED FROM full paper: Kapp GT, Liu S, Stein A, Wong DT, Remenyi A, Yeh BJ, Fraser JS, Taunton J, Lim WA and Kortemme T. Control of protein signaling using a computationally designed GTPase/GEF orthogonal pair. Proceedings of the National Academy of Sciences of the United States of America 109, 5277-82 (2012).